Basic Information

Symbol
hsa-mir-412
RNA class
miRNA miRNA hairpin
Alias
MIR412 MicroRNA 412 Hsa-Mir-412 Hsa-MiR-412-3p MIRN412 Hsa-Mir-154-P14_pre Hsa-MiR-412-5p MIMAT0026557 MIMAT0002170 MI0002464 Mir-412
Location (GRCh38)
14:101065447-101065537
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification
External database
HGNC NCBI Ensembl miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-412
Sequence length
91 nt
GC content
0.5275

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-34.30 kcal/mol
Thermodynamic ensemble
Free energy: -36.99 kcal/mol
Frequency: 0.0128
Diversity: 17.87
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
...((((((..((((((.((((.((((((.(((..((((((((....)))).))))))).)))))).)))).)))))))))))).......
Thermodynamic Ensemble Prediction
..,(((((({,({((((.((((.((((((.(({.,(((({{({....}))}.))))))).)))))).)))}.)))))))))))).,.....
Forensic Context

A study in human body fluids using microarray screening and RT-PCR validation found that the mir-412 was tested as a candidate for menstrual blood identification based on prior literature, but the results for menstrual blood were not replicated in this experimental validation [Zubakov et al. DOI:10.1007/s00414-009-0402-3]. A study in humans using massively parallel sequencing identified 90 absolute specific markers (ASMs) for body fluid/tissue identification, including the mir-412 as a potential marker for menstrual blood based on prior published data [Liu et al. DOI:10.3390/genes13091530]. An earlier review noted that the mir-412 was reported as differentially expressed in menstrual blood in a study by Hanson et al., which successfully created body-fluid specific assays for unambiguous identification [Courts & Madea DOI:10.1016/J.Forsciint.2010.07.002].