Basic Information

Symbol
hsa-mir-4325
RNA class
miRNA miRNA hairpin
Alias
MIR4325 MicroRNA 4325 Hsa-Mir-4325 MIMAT0016887 Hsa-MiR-4325 MI0015865
Location (GRCh38)
20:57321502-57321591
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Individual identification
External database
HGNC NCBI Ensembl miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-4325
Sequence length
90 nt
GC content
0.5000

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-27.50 kcal/mol
Thermodynamic ensemble
Free energy: -29.72 kcal/mol
Frequency: 0.7807
Diversity: 0.71
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
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Thermodynamic Ensemble Prediction
..,.{,....,,.|.|,,{((((({.(.((..(((((((..((((..((((....))))..))))..,,)}})))))..))).))))))}
Forensic Context

A study in humans demonstrated that the mir-4325 was differentially expressed and shared across five pairs of monozygotic twins, with its expression pattern validated by qRT-PCR [Xiao et al. DOI:10.1016/j.fsigen.2019.05.003]. The experimental findings, using miRNA microarray and qRT-PCR, showed differential expression in 9 of 18 twin pairs with a 2-fold change criterion and 11 pairs with a 1.5-fold change criterion, indicating its potential role as a biomarker for distinguishing genetically identical individuals in forensic identification.