Basic Information
Symbol
hsa-miR-4477b
RNA class
miRNA
mature miRNA
Alias
hsa-miR-4477b
MIR4477B
MicroRNA 4477b
Location (GRCh38)
-
Forensic tag(s)
Tissue/body fluid identification
External database
Sequence & Structure
Secondary Structure
Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-55.00 kcal/mol
Thermodynamic ensemble
Free energy:
-55.30 kcal/mol
Frequency:
0.4310
Diversity:
3.24
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
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Thermodynamic Ensemble Prediction
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Forensic Context
A study in humans using massively parallel sequencing identified the miR-4477b as an absolute specific marker (ASM) for semen, demonstrating its high specificity and expression in this body fluid [Liu et al. DOI:10.3390/genes13091530]. This miRNA is recognized as a specific marker for semen in forensic body fluid identification [Liu et al. DOI:10.1007/s00414-025-03656-2].