Forensic Context

A study in human tissues demonstrated that the miR-499a-3p exhibited high expression in pooled muscle samples but only a slight difference between heart and skeletal muscle, classifying it as a marker for muscle in general [Sauer et al. DOI:10.1016/j.fsigen.2017.02.002]. A study in humans demonstrated that the miR-499a-3p is a diagnostic biomarker for acute myocardial infarction (AMI), showing significant upregulation in AMI patients compared to non-AMI controls, with a qRT-PCR validation yielding an area under the curve (AUC) of 0.965 in receiver operating characteristic (ROC) analysis [Zhong et al. DOI:10.1002/jcla.23099]. In a separate human study investigating marathon-induced cardiac strain, the miR-499a-3p was studied as a potential diagnostic microRNA for myocardial infarction and showed no significant correlation with cardiac troponin T levels after strenuous exercise [Shirvani Samani et al. DOI:10.3390/jcm11010005].