Forensic Context

A review of human studies identified 197 miRNAs associated with wound healing, burn wound healing, and scarring, with specific expression patterns validated in human skin tissue and cell lines using qRT-PCR, microarrays, and next-generation sequencing [Siu et al. DOI:10.1111/wrr.13100]. A study in mice demonstrated that ethanol intoxication combined with burn injury significantly altered the mRNA and miRNA expression profile in intestinal epithelial cells, where miR-501-3p miR-501-3p was identified as a differentially expressed miRNA [Herrnreiter et al. DOI:10.1038/s41598-021-99281-1]. In human sepsis patients, RNA-sequencing and RT-qPCR validation identified miR-501-3p as significantly downregulated in peripheral blood, contributing to a panel of diagnostic biomarkers for the condition [Xu et al. DOI:10.3892/mmr.2022.12850].