Basic Information

Symbol
hsa-mir-5581
RNA class
miRNA miRNA hairpin
Alias
MIR5581 MicroRNA 5581 Hsa-Mir-5581 Hsa-MiR-5581-3p Hsa-MiR-5581-5p Hsa-Mir-5581_pre MIMAT0022275 MIMAT0022276 MI0019136
Location (GRCh38)
1:12191713-12191773
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification
External database
HGNC NCBI Ensembl miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-5581
Sequence length
60 nt
GC content
0.4833

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-21.30 kcal/mol
Thermodynamic ensemble
Free energy: -21.56 kcal/mol
Frequency: 0.6589
Diversity: 1.23
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
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Thermodynamic Ensemble Prediction
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Forensic Context

A study in humans using massively parallel sequencing identified the mir-5581-3p as an absolute specific marker for venous blood, demonstrating high specificity and expression in forensic stains [Liu et al. DOI:10.3390/genes13091530]. This miRNA was reported as a specific marker for venous blood in forensic body fluid identification [Liu et al. DOI:10.1007/s00414-025-03656-2].