Forensic Context

A study in humans demonstrated that mir-1273h-3p, identified via miRNA sequencing as differentially expressed across monozygotic twin pairs, was validated by both qPCR and droplet digital PCR (ddPCR) for distinguishing individuals, with ddPCR showing superior accuracy and mir-1273h-3p exhibiting no significant degradation in bloodstains stored at room temperature for 180 days or after 10 freeze-thaw cycles [Wang et al. DOI:10.1016/j.fsigen.2025.103230]. Separately, research in humans identified plasma exosomal hsa-miR-1273h-3p as part of an 18-miRNA panel with diagnostic potential for acute myocardial infarction, where its expression showed a downregulated pattern from healthy to coronary artery disease to AMI groups [Guo et al. DOI:10.21037/atm-20-2337]. A study in humans identified the mir-1273h-3p as significantly downregulated in the plasma of coronary artery disease patients compared to controls, with its expression profile validated by RNA sequencing [Zhong et al. DOI:10.1002/jcla.23020].