Basic Information

Symbol
hsa-mir-654
RNA class
miRNA miRNA hairpin
Alias
MIR654 MicroRNA 654 Hsa-MiR-654-5p Hsa-MiR-654-3p Hsa-Mir-654 MIRN654 Hsa-Mir-154-P23_pre MIMAT0003330 MIMAT0004814 MI0003676 Mir-654
Location (GRCh38)
9:70809975-70810084
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification
External database
HGNC NCBI Ensembl miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-654
Sequence length
81 nt
GC content
0.5309

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-28.30 kcal/mol
Thermodynamic ensemble
Free energy: -30.53 kcal/mol
Frequency: 0.0267
Diversity: 7.35
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
((((......((((.((((((.(.(((((.(((((((.........).))))))))))).).)))))).))))....))))
Thermodynamic Ensemble Prediction
((((......((((.((((((.,.((((,,(((((((,,....,,.),})))))))))).}.)))))).))))....))))
Forensic Context

A study in humans identified the mir-654 as a novel microRNA marker for vaginal secretion identification, demonstrating its high overexpression in vaginal secretions and high sensitivity and specificity with an area under the ROC curve of 0.81 when evaluated by qRT-PCR in additional body fluid samples [Park et al. DOI:10.1002/elps.201400075]. Another human study, which evaluated a range of candidate markers, noted that the mir-654 was removed from its final analysis due to low amplification efficiency in the target body fluid [Sauer et al. DOI:10.1016/j.fsigen.2016.01.018]. A study in humans demonstrated that the mir-654 is a specific marker for vaginal secretions and is used in a nine-miRNA-marker discriminant analysis model for identifying vaginal secretions, saliva, semen, menstrual blood, and peripheral blood [Song et al. DOI:10.1007/s00414-023-03091-1]. Another review confirms the mir-654 is mentioned as a specific marker for vaginal secretions in forensic body fluid identification [Liu et al. DOI:10.1007/s00414-025-03656-2].