Forensic Context

A study in humans demonstrated that the miR-654-3p is down-regulated in myocardial tissue from deceased septic patients compared to non-septic controls [Pasi Lehto et al. DOI:10.1038/s41598-024-81114-6]. A study in humans demonstrated that the miR-654-3p was evaluated as a candidate marker for vaginal secretion identification but showed no expression or Ct values >35 in the target fluid and was consequently discarded from the final panel [Altmeyer et al. DOI:10.1002/elps.202400089]. In contrast, a review of forensic applications notes that miR-654-5p, a closely related isoform, is part of a nine-miRNA-marker discriminant analysis model for identifying vaginal secretions, saliva, semen, menstrual blood, and peripheral blood [Song et al. DOI:10.1007/s00414-023-03091-1].