Forensic RNA Knowledge Base

Basic Information

Symbol

hsa-miR-661

RNA class

miRNA mature miRNA

Alias

hsa-miR-661 MIR661 MicroRNA 661

Location (GRCh38)

Forensic tag(s)

Tissue/body fluid identification Wound age identification Other applications

External database

miRBase

RNAcentral

Sequence & Structure

Transcript ID

hsa-miR-661

Sequence length

24 nt

GC content

0.7083

Secondary Structure

Generated by RNAfold

Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.

Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.

Minimum free energy

-49.30 kcal/mol

Thermodynamic ensemble

Free energy: -49.73 kcal/mol

Frequency: 0.4954

Diversity: 2.87

MFE Structure Visualization

Structure Prediction

MFE Structure Prediction

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Thermodynamic Ensemble Prediction

.....((((..(((.(((((((.(((((((((.((((((.(((........))).))).))).))))))))).)).))))).)))))))

Forensic Context

A review of human studies identified 197 miRNAs associated with wound healing, burn wound healing, and scarring, with specific miRNAs like the miR-661 showing altered expression in human skin tissue post-burn and in hypertrophic scar tissue compared to normal skin [Siu et al. DOI:10.1111/wrr.13100]. A separate forensic study in humans established a decision algorithm using four miRNAs, including hsa-miR-891a-5p for semen and hsa-miR-144-3p for blood, which correctly identified 9 out of 10 blinded body fluid samples and was effective on aged specimens [Sauer et al. DOI:10.1016/j.fsigen.2016.01.018]. A study in humans demonstrated that miR-661 was identified as a potential target of exo-circ-0020887 and exo-circ-0009590 through bioinformatics prediction [Wang et al. DOI:10.1515/med-2023-0807].