Basic Information

Symbol
hsa-mir-664b
RNA class
miRNA miRNA hairpin
Alias
MIR664B MicroRNA 664b Hsa-Mir-664b Hsa-MiR-664b-3p MicroRNA 644b MIR644B Hsa-MiR-664b-5p Hsa-Mir-644b MIMAT0022272 MIMAT0022271 MI0019134 Mir-664b
Location (GRCh38)
X:154768596-154768656
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification
External database
HGNC NCBI Ensembl miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-664b
Sequence length
61 nt
GC content
0.4262

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-24.80 kcal/mol
Thermodynamic ensemble
Free energy: -25.67 kcal/mol
Frequency: 0.2447
Diversity: 2.46
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
((((((..(((((.(((.(((((((((.......))))))))).))).)))))))))))..
Thermodynamic Ensemble Prediction
((((((..(((((.{((.(((((((((.......))))))))).))}.)))))))))))..
Forensic Context

A study in humans using massively parallel sequencing identified the mir-664b-3p as an absolute specific marker for venous blood, demonstrating high specificity and expression in forensic body fluid identification [Liu et al. DOI:10.3390/genes13091530]. This research, which characterized small RNA profiles in six forensically relevant body fluids/tissues, established the mir-664b-3p as a stable and specific RNA marker for distinguishing venous blood from other biological samples in forensic genetics [Liu et al. DOI:10.1007/s00414-025-03656-2].