Forensic Context

A review of human studies identified 197 miRNAs associated with wound healing, burn wound healing, and scarring, with specific miRNAs like hsa-miR-21 and hsa-miR-31 upregulated in hypertrophic scar tissue and shown to promote keratinocyte proliferation and migration [Siu et al. DOI:10.1111/wrr.13100]. A separate forensic study in humans established a multi-class support vector machine model using 12 miRNA markers, including hsa-miR-451a and hsa-miR-205-5p, which achieved 98% accuracy in body fluid identification from single-source samples and demonstrated efficacy with aged casework samples [Li et al. DOI:10.1016/j.fsigen.2024.103180]. A study in mice demonstrated that the miR-708-5p was the most up-regulated miRNA in an arrhythmogenic cardiomyopathy (ACM) mouse model heart, where it was predicted to be involved in regulating Wnt/β-catenin signaling [Lou et al. DOI:10.1016/j.jflm.2022.102332]. In a separate investigation using primary mouse cardiomyocytes, the miR-708-5p exhibited a pattern of decreased expression under hypoxic conditions at 37°C and increased expression under therapeutic hypothermia at 32°C, indicating its role in the cellular response to hypoxia and cardioprotection [Xue et al. DOI:10.1161/JAHA.122.026160].