Forensic Context

A study in severely injured human patients identified 69 differentially expressed microRNAs, with the let-7i showing direct correlations with arterial blood gas HCO3 and base deficit [Uhlich et al. DOI:10.1016/j.surg.2014.06.017]. In young human patients with acute coronary syndrome, the let-7i was significantly down-regulated in ST-segment elevation myocardial infarction and demonstrated high discriminatory power as a diagnostic marker [Tong et al. DOI:10.3390/ijms19051467]. A study in human body fluid stains demonstrated that the let-7i serves as a stable endogenous reference for normalization in RT-qPCR assays, showing resilience across various compromising treatments including heat, UV, chemicals, and environmental exposure [Layne et al. DOI:10.1111/1556-4029.14113]. Further developmental validation in humans confirmed its utility within a six-miRNA panel, where normalization using the let-7i and let-7g enabled a quadratic discriminant analysis model to classify seven forensically relevant body fluids with 93.3% overall accuracy [Rhodes et al. DOI:10.2692].