Basic Information

Symbol
hsa-mir-135a-1
RNA class
miRNA miRNA hairpin
Alias
MIR135A1 MicroRNA 135a-1 Hsa-MiR-135a-3p Hsa-MiR-135a-5p Hsa-Mir-135a-1 Hsa-Mir-135-1 MicroRNA 135-1 MIRN135-1 MIRN135A1 Hsa-Mir-135-P1_pre MIMAT0004595 MIMAT0000428 Mir-135a-1 MI0000452 hsa-mir-135a
Location (GRCh38)
4:112648183-112648251
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification
External database
HGNC NCBI Ensembl miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-135a-1
Sequence length
90 nt
GC content
0.5222

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-44.10 kcal/mol
Thermodynamic ensemble
Free energy: -45.04 kcal/mol
Frequency: 0.2172
Diversity: 2.44
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
...(((((((((.(.((((((((((..((((((((((((.............)))))))))))).)))))))))).).)))))))))...
Thermodynamic Ensemble Prediction
...(((((((((.(.((((((((((..((((((((((((.............)))))))))))).)))))))))).).))))))))).}.
Forensic Context

A study in humans demonstrated that the mir-135a is a highly specific and stable marker for semen identification, as quantitative RT-PCR confirmed its strong over-expression in semen relative to other forensically relevant body fluids [Zubakov et al. DOI:10.1007/s00414-009-0402-3]. This specificity was replicated in subsequent research, which also reported that the mir-135a displayed distinctly higher expression levels in semen compared to other body fluids [Sauer et al. DOI:10.1016/j.fsigen.2016.01.018]. A study in humans demonstrated that the mir-135a is a specific marker for seminal fluid identification and spermatogenesis, showing a significant expression increase in asthenospermia and oligospermia samples but decreased expression in astheno-oligospermia and normal semen [Mawlah et al. DOI:10.5455/aim.2022.30.205-212].