Basic Information

Symbol
hsa-mir-135b
RNA class
miRNA miRNA hairpin
Alias
MIR135B MicroRNA 135b Hsa-MiR-135b-3p Hsa-MiR-135b-5p Hsa-Mir-135b MIRN135B Hsa-Mir-135-P4_pre MIMAT0004698 MIMAT0000758 MI0000810 Mir-135b
Location (GRCh38)
1:205448302-205448398
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification
External database
HGNC NCBI Ensembl OMIM miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-135b
Sequence length
97 nt
GC content
0.5258

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-46.70 kcal/mol
Thermodynamic ensemble
Free energy: -48.34 kcal/mol
Frequency: 0.0698
Diversity: 9.54
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
..(((((((((((((((((((((((...(((((((((((((......)))..))))))))))..))))))))))))))))))).)))).........
Thermodynamic Ensemble Prediction
..((({(((((((((((((((((((...(((((((((({((......))}..))))))))))..))))))))))))))))))),))}|,........
Forensic Context

A study in humans demonstrated that the mir-135b is strongly over-expressed in semen relative to other forensically relevant body fluids, confirming its semen specificity [Zubakov et al. DOI:10.1007/s00414-009-0402-3]. Subsequent research in humans confirmed this specificity and high abundance in normal semen but found its expression was significantly down-regulated and dispersed in various infertile semen samples, including asthenospermia, oligospermia, azoospermia, and combined conditions [Tian et al. DOI:10.1016/j.fsigen.2017.12.010]. A study in humans demonstrated that the mir-135b showed remarkably high expression, especially in Astheno-Oligospermia (ASOS) at approximately 140 gene folds, serving as a strong marker for distinguishing this infertility condition [Mawlah et al. DOI:10.5455/aim.2022.30.205-212]. Another study mentioned the mir-135b as a semen marker identified in prior research, supporting its role in body-fluid identification [Courts & Madea DOI:10.1111/J.1556-4029.2011.01894.X].