Forensic Context

A study in human postmortem brain tissue demonstrated that the mir-16 was significantly upregulated in traumatic brain injury (TBI) cases compared to non-traumatic controls and its expression was significantly higher in cases with short survival (<1 day) and in the critical–fatal TBI subgroup, indicating its potential as a diagnostic and prognostic biomarker for TBI severity and survival time estimation [Consalvo et al. DOI:10.3390/ijms25179539]. Separately, research on human cadaver serum established that exosomal the mir-16 remains stable for at least three days when stored at or below 20°C, validating its reliability for postmortem molecular analysis in forensic contexts [Kanno et al. DOI:10.1007/S00414-022-02913-Y]. In a separate experimental model using Wistar rats, the mir-16 was found to be downregulated in both corpus cavernosum tissue and peripheral blood in a group with combined diabetes and alcohol consumption, which exhibited the greatest endothelial injury, and plasma levels showed high diagnostic accuracy (AUC >0.9) for detecting this endothelial dysfunction [Tiraboschi et al. DOI:10.1016/j.esxm.2021.100326].