Forensic Context

A study in young human patients with acute coronary syndrome (ACS) identified the miR-101-3p as up-regulated in ST-segment elevation myocardial infarction (STEMI) patients via small RNA sequencing [Tong et al. DOI:10.3390/ijms19051467]. A separate forensic investigation in human cadavers demonstrated that serum exosomes and their miRNA cargo, including the miR-101-3p, remain stable for at least three days when stored at or below 20°C, and subsequent miRNA profiling of postmortem serum from acute myocardial infarction cases revealed significant dysregulation of numerous exosomal miRNAs [Kanno et al. DOI:10.1007/S00414-022-02913-Y]. A study in humans demonstrated that plasma levels of the miR-101-3p were significantly upregulated in patients with Adult-onset Still's disease (AOSD) compared to healthy controls and sepsis patients [Hu et al. DOI:10.3389/fimmu.2018.03099]. The miR-101-3p was part of validated diagnostic panels, correlated with clinical symptoms and pro-inflammatory cytokines, and effectively differentiated AOSD from sepsis with high sensitivity and specificity.