Basic Information

Symbol
hsa-mir-203b
RNA class
miRNA miRNA hairpin
Alias
MIR203B MicroRNA 203b Hsa-Mir-3545 MicroRNA 3545 MIR3545 Hsa-MiR-203b-5p Hsa-MiR-203b-3p Hsa-Mir-203b MIMAT0019813 MIMAT0019814 MI0017343
Location (GRCh38)
14:104117418-104117503
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification
External database
HGNC NCBI Ensembl miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-203b
Sequence length
86 nt
GC content
0.5465

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-38.20 kcal/mol
Thermodynamic ensemble
Free energy: -40.06 kcal/mol
Frequency: 0.0492
Diversity: 7.76
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
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Thermodynamic Ensemble Prediction
((((..((.((((((((((((.((.((((.((((((.{(.,......),,.)),}))).)))).)).)))))))))))).))))))
Forensic Context

A study in humans using whole miRNome massively parallel sequencing identified the mir-203b-5p as one of nine selected miRNA markers for body fluid identification, where its presence helped distinguish between semen and vaginal secretion samples based on regression coefficients in a model with a cross-validation accuracy of 0.93 [Dørum et al. DOI:10.1016/j.fsigen.2019.02.015]. A separate review also lists the mir-203b-5p as a specific marker for vaginal secretions, highlighting the broader forensic application of RNA markers for body fluid identification based on specificity and stability [Zhiyong Liu et al. DOI:10.1007/s00414-025-03656-2].