Forensic Context

A study in humans demonstrated that miR-29a-5p was downregulated four days post-burn in deep partial thickness burn skin compared to normal skin [Siu et al. DOI:10.1111/wrr.13100]. In a separate human and mouse study, miR-29a-5p was markedly upregulated in trauma plasma and exhibited strong diagnostic and predictive abilities for overall trauma severity, organ injury, coagulation, endothelial activation, and inflammation, with a cross-validated AUROC of 0.98 for distinguishing trauma from healthy controls [Ren et al. DOI:10.1016/j.isci.2025.113569]. A study in mice demonstrated that the miR-29a-5p is downregulated in paraquat-treated murine lung alveolar epithelial cells (MLE-12 cells) and its target genes, including AGO2, TET3, and TET1, are involved in DNA methylation and apoptosis pathways [Zhao et al. DOI:10.2131/Jts.45.423]. In the context of ionizing radiation injury, the miR-29a-5p is reported as a biomarker for dose estimation and organ injury, with its expression downregulated in human plasma after radiotherapy and involved in predicting radiation-induced conditions in mice and non-human primates [Jia et al. DOI:10.3389/fcell.2022.861451].