Basic Information

Symbol
hsa-mir-320a
RNA class
miRNA miRNA hairpin
Alias
MIR320A MicroRNA 320a Hsa-Mir-320 MicroRNA 320 Hsa-MiR-320a MIRN320A MIRN320 Hsa-Mir-320-P1_pre Hsa-MiR-320a-5p Hsa-MiR-320a-3p Hsa-Mir-320a MIMAT0037311 MIMAT0000510 MI0000542 Mir-320a
Location (GRCh38)
17:48632490-48632559
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification Sudden cardiac death diagnosis Drug abuse diagnoses
External database
HGNC NCBI Ensembl OMIM miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-320a
Sequence length
72 nt
GC content
0.5972

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-41.80 kcal/mol
Thermodynamic ensemble
Free energy: -42.44 kcal/mol
Frequency: 0.3557
Diversity: 3.48
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
.(((....(((((((((..(((((((.((((((....)))))).)))))))..))))))))).....)))..
Thermodynamic Ensemble Prediction
.{((..,.(((((((((..(((((((.((((((....)))))).)))))))..))))))))).....))}..
Forensic Context

A study in human plasma from young acute coronary syndrome patients identified the mir-320a as down-regulated in STEMI patients via small RNA sequencing and used it as a reference miRNA for qRT-PCR normalization [Tong et al. DOI:10.3390/ijms19051467]. A study in humans demonstrated that the mir-320a is significantly upregulated in the plasma of heroin-dependent patients compared to healthy controls, with an AUC of 0.748, sensitivity of 71.9%, and specificity of 76.1% for diagnosing heroin use disorder [Liu et al. DOI:10.3389/fpsyt.2021.679206]. In a separate forensic investigation, the mir-320a was validated as the most stable endogenous reference gene for miRNA expression analysis across five forensically relevant body fluids (peripheral blood, menstrual blood, saliva, semen, and vaginal secretions) and in degraded samples, with its combination with RNU6b providing the most stable normalization effect for body fluid identification [Wei et al. DOI:10.1016/j.fsigen.2023.102827].