Basic Information

Symbol
hsa-mir-324
RNA class
miRNA miRNA hairpin
Alias
MIR324 MicroRNA 324 Hsa-Mir-324 MIRN324 Hsa-Mir-324_pre Hsa-MiR-324-5p Hsa-MiR-324-3p MIMAT0000762 MIMAT0000761 MI0000813 Mir-324
Location (GRCh38)
17:7223297-7223379
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Forensic psychiatry evaluation Sudden cardiac death diagnosis Chronological age estimation Other applications
External database
HGNC NCBI Ensembl miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-324
Sequence length
83 nt
GC content
0.6145

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-36.80 kcal/mol
Thermodynamic ensemble
Free energy: -37.97 kcal/mol
Frequency: 0.1495
Diversity: 8.68
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
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Thermodynamic Ensemble Prediction
..(((((((.(((((.(((.(.(((.(((((.(((.{(..........))))).))))).))).).))).))))).}))))))
Forensic Context

A study in humans demonstrated that hsa-miR-324 was significantly down-regulated in the prefrontal cortex of depressed suicide subjects compared to non-psychiatric controls [Smalheiser et al. DOI:10.1371/journal.pone.0033201]. In a separate human study of severe trauma, the mir-324 showed a direct correlation with TLR3 gene expression in the whole blood of severely injured patients [Uhlich et al. DOI:10.1016/j.surg.2014.06.017]. A study in humans demonstrated that the mir-324 is significantly upregulated in patients with plaque rupture compared to those with plaque erosion in ST-segment elevation myocardial infarction, where a diagnostic model combining it with two other miRNAs achieved an area under the curve of 0.768 in an independent test set [Li et al. DOI:10.1016/j.ygeno.2020.11.019]. Separately, a forensic investigation in humans established that the mir-324 is negatively correlated with aging in blood samples, and its combination with other small RNAs in a gradient boosting model enabled age estimation with a mean absolute error of 3.171 years [Fang et al. DOI:10.1007/s00414-023-03028-8].