Basic Information

Symbol
hsa-mir-335
RNA class
miRNA miRNA hairpin
Alias
MIR335 MicroRNA 335 Hsa-MiR-335-3p Hsa-MiR-335-5p Hsa-Mir-335 MIRN335 Hsa-Mir-335_pre MIMAT0000765 MIMAT0004703 MI0000816 MiRNA335 Mir-335
Location (GRCh38)
7:130496111-130496204
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Sudden death from CNS diseases Forensic psychiatry evaluation Cause of death analysis Other applications
External database
HGNC NCBI Ensembl OMIM miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-335
Sequence length
94 nt
GC content
0.3830

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-40.20 kcal/mol
Thermodynamic ensemble
Free energy: -41.42 kcal/mol
Frequency: 0.1376
Diversity: 5.35
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
.....((((.((((((((.(((((((((.((((((((.((......)).)))))))).))))))))).)))))))).)))).............
Thermodynamic Ensemble Prediction
.,,..((((.((((((((.(((((((((.(((((((({((,...,})).)))))))).))))))))).)))))))).))))...,,........
Forensic Context

A study in humans demonstrated that the mir-335 is significantly upregulated in plasma from patients with acute spinal cord injury (SCI) at emergency room admission compared to healthy controls, as validated by droplet digital PCR [Hörauf et al. DOI:10.3390/ijms262210954]. In a separate human postmortem study, the mir-335 was found to be significantly decreased in the prefrontal cortex of depressed suicide subjects compared to non-psychiatric controls, with its validated targets including SOX4, PTPRN2, and MERTK [Smalheiser et al. DOI:10.1371/journal.pone.0033201]. A study in rhesus macaques (Macaca mulatta) demonstrated that the mir-335 was identified as a common radiation response marker, being one of the ten most statistically significant miRNAs among the 76 common to both genders and both doses at any time point following whole thorax irradiation [May et al. DOI:10.1038/s41598-022-16316-x]. In a separate study using Sprague-Dawley rats, the mir-335 was found to work with miR-182 and miR-484 to decrease the neu2 gene in response to hindlimb unloading [Song et al. DOI:10.1097/BCR.0000000000000444].