Basic Information

Symbol
hsa-mir-409
RNA class
miRNA miRNA hairpin
Alias
MIR409 MicroRNA 409 Hsa-MiR-409-5p Hsa-MiR-409-3p Hsa-Mir-409 MIRN409 Hsa-Mir-154-P36_pre MIMAT0001638 MIMAT0001639 MI0001735 Mir-409
Location (GRCh38)
14:101065300-101065378
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Wound age identification Cause of death analysis Chronological age estimation Individual identification
External database
HGNC NCBI Ensembl OMIM miRBase RNAcentral

Sequence & Structure

Transcript ID
hsa-mir-409
Sequence length
79 nt
GC content
0.5316

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-36.70 kcal/mol
Thermodynamic ensemble
Free energy: -37.86 kcal/mol
Frequency: 0.1521
Diversity: 4.68
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
((((((.(((((((.((((..((((((((((((((........).)))).)))))))))..)))).)))))))))))))
Thermodynamic Ensemble Prediction
{(((((.(((((((.((((.,((((((((((((((........).)))).))))))))).,)))).))))))))))))}
Forensic Context

A review of human studies found that the mir-409-3p is downregulated 24 hours post-injury in skin wounds compared to unwounded skin [Siu et al. DOI:10.1111/wrr.13100]. A study in mice demonstrated that the mir-409-3p was upregulated in liver tissue following whole-body ionizing radiation exposure, with a log2 fold change of 1.19, as identified via high-throughput deep sequencing and validated by consistent qRT-PCR results [Lu et al. DOI:10.1002/cbin.10627]. In human monozygotic twins, longitudinal analysis over eight years identified the mir-409-3p as a significant predictor of age-related functional decline, where a higher baseline plasma level was associated with improved physical functionality over time, as indicated by a positive beta coefficient [La Grotta et al. DOI:10.1016/j.mad.2025.112099].