Basic Information

Symbol
CST5
RNA class
mRNA
Alias
Cystatin D Cystatin-D Cysteine-Proteinase Inhibitor Cystatin 5 Cystatin-5
Location (GRCh38)
20:23875934-23880081
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification
External database
HGNC NCBI Ensembl OMIM UniProt GTEx

MANE select

Transcript ID
NM_001900.5
Sequence length
755.0 nt
GC content
0.5576

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-277.50 kcal/mol
Thermodynamic ensemble
Free energy: -288.52 kcal/mol
Frequency: 0.0000
Diversity: 208.33
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
((((((((((...((((((((.((((.((.(((((((((.((..(((.....)))..)).)))))).))).))(((.((((.....(..(((((((((....((((((....(((.(((.(.(((((.((((....).))).)))))).))).)))((((((((.(((((((((((.(((((.((((((........))).)).).))).))..))))...))))))).))........))))))((((......)))).(((...((((((((((....))).).))))))..)))))))))......)))))))))..)..)))).)))(((((..((((....))))))))).((((...((((((........((((....))))(((((.(((.(((......))).....((((((((...))))))))....))).)))))....))))))(((.......))).(((((.(((...)))..)))))(((((((((((..(.((((((...((((...))))..))).((((...........))))))).)..))))))))))))))).((((((...))))))..(((((((..(((....)))..))))))))).))))))))))))))))))))(..(((((((..((((((((....((....))............(((......)))...(((((((...)))))))......))))))))........)))))))..)..
Thermodynamic Ensemble Prediction
((((((((((...((((((((.(({{.{(.(((((((((.((..(((.....)))..)).)))))).))).)}.((({.((((...((((,((({,({((.{((((({.,..{{,.|,,}|}|||,|}|||,}}}.)})),.,))))}.))).|}|{{{{((((,((((((({(({.({(((.,(((((........))).)).}.))).)}..})))...})))))).))........}}}})||,{{.....,|,,{((({{{{(((((((,,((({{.(({|..{|,,,}}}))).))))}..)}})||||{{{(,,,,.,,{{,{{,,,|||,}|||||||.},}))}}}}.,,|,..,((((((....,||.((((....)))){{{{{.{||,{{{......)))...,.((((((((...))))))))}...}}}.}})))|,..)))))}(((.......))).{{(({.{{{...}))..)))))|||,,{((((({|{|({|.||..}}|}}},..)))}.))).,|||.,}}}}},,}})))))))}}{|||{,|,||...))))}((((({...))))))..(((((((..(((....)))..)))))))}).)))))))))))))))))))){..(((((((..((((((((....({....))............(((......)))...(((((((...)))))))......))))))))........)))))))..}..

Transcripts

ID Sequence Length GC content
NM_001900.5 NCBI
GCUUCCUGCCUCAGCCUUCUGCUGCUUUGCUCUCUGGGAGAUCCAGCUC… 755 nt 0.5576
Summary

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein found in saliva and tears. The encoded protein may play a protective role against proteinases present in the oral cavity. [provided by RefSeq, Jul 2008]

Forensic Context

A study in humans demonstrated that Cystatin-D exhibited a Gini impurity score of 0, was present in most pure saliva samples and in mixtures containing saliva, and was absent in mixtures without saliva, confirming its role as a discriminatory protein for saliva classification in a proteomic workflow using LC-MS/MS [Shehata et al. DOI:10.1016/j.fsigen.2025.103343].