Basic Information

Symbol
ENO1
RNA class
mRNA
Alias
Enolase 1 Alpha-Enolase ENO1L1 MBP-1 MPB1 PPH ENO1 Intronic Transcript 1 (Non-Protein Coding) 2-Phospho-D-Glycerate Hydro-Lyase Plasminogen-Binding Protein ENO1 Intronic Transcript 1 Phosphopyruvate Hydratase Enolase 1, (Alpha) Non-Neural Enolase EC 4.2.1.11 ENO1-IT1 NNE Epididymis Secretory Protein Li 17 C-Myc Promoter-Binding Protein-1 MYC Promoter-Binding Protein 1 C-Myc Promoter-Binding Protein Alpha Enolase Like 1 Tau-Crystallin Enolase-Alpha HEL-S-17 EC 4.2.1 MBPB1 MPB-1
Location (GRCh38)
1:8860977-8879289
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Other applications
External database
HGNC NCBI Ensembl OMIM UniProt GTEx

MANE select

Transcript ID
NM_001428.5
Sequence length
1781.0 nt
GC content
0.5441

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-654.20 kcal/mol
Thermodynamic ensemble
Free energy: -686.37 kcal/mol
Frequency: 0.0000
Diversity: 598.58
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
(((.((((((((..(((((((..((((((((((.(.(((..............(((((.....)))))...(((((........)))))...((((...((((((...)).))))...))))...............))).).))))))))))...))).))))...)))))..(((((((.((.....)))))))))..(((((..((((((((.(((.(((((..((((((......))))))..)).)))..))))))))........)))..))))).((((..(((((..((((((.....((..((((((.....))))))...))...)))))).)))))..)))).))).)))....(.((((((((...................((((((((((.(((((..............))))).....(((((((((.((((..(((((((.((....((.(((((...(((((((((....(((..(((((((....((((((((((((..(((.((((((((((....((((((((..(((((((.((.(((.((((..........)))).)))(((((((((((((.....))))).)))).))))((((.(((((..((((((..(((((((..((.(((((..(((..(.((((((((.(((....((..(((...(((((.((...)).)).))).......)))..))..)))..)))))))).).((((((((((((((((((((((....((((((((((...(.(((((.(..((((((((.((((((((((((...((....))...)))))..))))))).)))))).....))..)))))).)..))))))))))..)))).)))).))))))..(((((.(((((((((((.(((((((.....(((...(((((.((((((.((((.((((....(((((((....))))))).((((((.((.(((.((((((....))))))..))))).))))))((((((((.......(((((((((((((((((.....(((((((.(((((..((((.((((((((..........)).))))........))...)))).))))).).))))))))))))..............)))))))..))))))))).)))..)))))).)).))))))..)))))..))))))))))))))((((((...))))))....))))))))))))...)))))))))))..)))))..))...)))))))))))))..)))))(((.((((.......)))).)))))))((((((((..((....((((((((((.........))))))))))...))..))))))))..)).)))))))))))))))....))..)))))))).))))))))(((((...(((....)))..)))))))).))))..)))))))...))))))))))))))))).))..)).)))))))......(((.(((((....))))).)))..(((......))).)))).)))))))))(((((..((((...))))..)))))...((((.(((((((((....))..)))))))..(((((....))))).((((....))))........(((....(((((......)))))....)))......)))))))).)))))).(((((((((((...)))))...))))))..)))))))))..............((((........))))....
Thermodynamic Ensemble Prediction
....{(((((((({({{(((({,((((({,{{(((.((({{{{{{,.,,.,,,(((({..,.,||}||,...||,..,,,,,,,{(((((.(((({,((((((({....,{{{.{{{{{({,.{{{{{..{{{,{,{,,{,(({{({{{{{{|.|||||{||||..,|||.{{.(((((({,((.....))))))))).})(((({.,,,(((((.(((.(((((..((((((......))))))..)).))).,,}}))))).}}|}}...,....,.{{.((((..(((((..((((({...,.,{..((((((.....))))))...}).,,)))))).)))))..)))).,}...{{{...,{,,,,,....,,.,.{{{||......{||,|,{||,,.,,,))).}..)}}......,}))},{(,,(({(({,..|||||..||(((((,,((...,(({{{(((({.{(((((((({(,((((,,(((({,,,,|||{{{((((((((,,(((,{{{{((((((,,..({(({{{{..{{{||||,|}},|||||{,...,|,,...,,})}))}||||||{{{{|||.....)))))),}))})))||,,{.{|||{,,|||||{.,|||||{|,,|||{{,||,}{{||.||||,..,||,})},,))}}}.|{,...||||{.{..,,,},))..,),,,.,,,}}},}}...,))}}),,,,|||(({((((((({((((((((((((((....((((((((((...(.(((((.(..{(((((((.((((((((((((...{{....}}...))))),.))))))).))))))....,}}..}))))),}..))))))))))..)))),,))).))))))..|||||.{||||||((((.(((((((.....{{{...(((((.((((((.((((.((((....(((((((....))))))).((((((.((.({{.((((((....))))))..,),)).))))))((((((((.......(((((((((({((((({....,(((((((.(((((..((((.,.{({,{{...,......)).}}||....,,.})),..)))).))))).),}))))))))))).....,,,.....,)))))))..))))))))).)))..)))))).)).))))))..)))))..}}}))))))))))),|||{|,,,)})))),.,,)))))}}|||||...})))))}},}}.,},}))}.)}}},))))))))))))),,}}||}.||.||||}},,..})}})}},)}}|||(((((((,,{(,,..((((((((((.........))))))))))...||,,||||||||.,|||||||||}}||}|||}....}|,.}}}}}}}}.}|}||}}||||{{...{{{..,,|||..||||,,||{|{{{{,||}}}},...}},)))),)))))))),.)).,))}))}})),.},.,}|||.|||((|,,.}}))|,}}}.,|||,.,,,,,}}.|}||,|||}},,,,(((((..((({...))))..))))),,}}|}},}}))))}})))}})))))))})))))))))|}.,,}))),,})))}},)))}}}}}..}}}|||}}}}(((({......}))))..,,))),,,,)))))}},})}),.||}}||||||||{||,,,))))}...}})}}}.||{{{{|,,,,}...,,....,}}},|,},,})))}),))}...

Transcripts

ID Sequence Length GC content
NM_001201483.4 NCBI
AGUAUCUGUGGGUACCCGGAGCACGGAGAUCUCGCCGGCUUUACGUUCA… 3161 nt 0.5302
NM_001353346.3 NCBI
AGUAUCUGUGGGUACCCGGAGCACGGAGAUCUCGCCGGCUUUACGUUCA… 1781 nt 0.5452
NM_001428.5 NCBI
AGUAUCUGUGGGUACCCGGAGCACGGAGAUCUCGCCGGCUUUACGUUCA… 1781 nt 0.5441
Summary

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

Forensic Context

A study in humans analyzing peripheral blood transcriptomes from burn patients identified the ENO1 as a key lactylation-related molecule with an AUC of 0.919 for distinguishing burn patients from controls [Li et al. DOI:10.3389/fmed.2025.1554791]. Another study in humans and mice identified the ENO1 as a top hub gene for CD38high monocytes, which are a diagnostic biomarker for sepsis, and found it to be part of a hyperactivated glycolysis signature in this pathogenic monocyte subset [Hua et al. DOI:10.1002/advs.202500457]. A study in mice demonstrated that the ENO1 gene (ENO1) was differentially expressed in blood leukocytes at 2 hours post-injury, showing a model-specific pattern where it was downregulated in burn injury but upregulated in both LPS infusion and trauma/hemorrhage models [Brownstein et al. DOI:10.1152/physiolgenomics.00213.2005]. In a separate investigation in rats, the ENO1 (Eno1) was identified as a downregulated metabolism gene involved in glycolysis in liver tissue on day 1 following a 20% total body surface area burn injury [Jayaraman et al. DOI:10.1016/j.jss.2007.05.025].