Basic Information

Symbol
KRT20
RNA class
mRNA
Alias
Keratin 20 K20 CK20 Keratin, Type I Cytoskeletal 20 Keratin 20, Type I Protein IT MGC35423 CK-20 Cytokeratin 20 Cytokeratin-20 Keratin-20 KRT21 CD20
Location (GRCh38)
17:40874934-40885243
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Tissue/body fluid identification
External database
HGNC NCBI Ensembl OMIM UniProt GTEx

MANE select

Transcript ID
NM_019010.3
Sequence length
1804.0 nt
GC content
0.4579

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-508.60 kcal/mol
Thermodynamic ensemble
Free energy: -541.93 kcal/mol
Frequency: 0.0000
Diversity: 577.87
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
...................((....(((((..((((.((((((..(((((((((.....)))))))))...(((..(((((....))))))))(((((.((.(((((.(((((((((.....))))).)))).)))))(((((.((((((.(.((((..((((((.(..(((((((((...((((.((....))))))..)))))).))).).))))))..)))).))).))))..)))))..((((.((..(((((((((.((......)).)))).)))))...........(((...))).((.((((.....)))))))).))))((((........))))...(((((........)))))..(((((.((..((((..((((...))))))))..))))))).(((.....))).........)).)))))((((..(((((...(((((((......)))......(((((((..((((((((((((((..(((((.((((((((((..(((((((..((((.(.(((.(((((.(((.(((.....)))..((((....))))(((((.((((((((....(((((......))))).................((((((...((((...))))...)))))).........(((((.........)))))....(((((..(((..((((((....((((.(((((((....(((((.((((.((((((.(.(((......))).).)))))).))))..((((.......)))))))))...((((....))))))))))).)...)))....)))))).....(((((((.....)))))))(((((((..............((((....))))...........))))))).(((((........)))))..)))...)))))))).))))))))))....)))))))).))))))))..))))....(((((((((....)))))))))............))).)))))))))))))))...(((((((..(((....)))...))))))).......((((...(((((..((....(((....)))..))..)))))....))))..((((......)))).......((((.((((..((((((((..(((((.((((((((.......(((.(((.......((((((.(((((..((((((((((.((....((((((.......(((.....(((...(((.(((((.((........)).)))))...)))..))).(((........))))))........))))))......)).))))..))...))))))))).)))))).........(((((....(((......(((.....))))))....))))).................)))))).......)))))))))))))))))))))...)))).)))).(((.((.(((((((((...(((((((((((((((..(((((.(((...)))..))..........)))..))))...)))))))))))............(((..((........)).)))..))))))))))))))(((.....))).))))))))))))))..)))...))))...(((((((.........(((((.....((((........))))......)))))))))))).....................))))...))))).))))............)))))).)))))))))....))..(((.....))).
Thermodynamic Ensemble Prediction
.,,,..,,{{{{{(,{,..{{,,,{({.((,,{((({{(((((..(((((((((.....)))))))))..{({{..(((((....))))),}},||,{,,,.,((((.(((((((((.....))))).)))).))))|(((((.((((((.(.((((..((((((.(..(((((((((...((((,,,..,,.|}}}}..)))))).))).}.))))))..)))).})).))))..}))|||{|||||||||({{({((((.{(......)}.)))).}}}}|.,,.....,.|||{{{{|.|.,,.,|||}},..||||,||{,,||(((((,,,{.{{,|||||..{((((,,,,....}}}}|,.(((((.((..((((..((((...))))))))..))))))).,|||{,,{{.{{{.....,{{{,{..|||||.,(((((.,,.(({(({{,{,,.,(({..,|||...|.))))||)|||..|}}},.,,{||||,||||||{{{{,}{{|||||,.|||,||}|||.{{{((.(((({(....,.}}}..((((....}}}|((((((((({{(((....(((((......)))))..............}}|,|||||}}}|||}..}},.,...||}.....}},|,|.,{|({{{{.....,))))}..,,||{{|..|||..|{((((....,{{{.{{{|||{..,|||.||,,,||}|||{||}{.|||,,,,,}))},),}))))).))|||,{{{{...,,..}))})))}}..,|.||..,,},,,}||||||{|...}||,,,,||||||....,(((((({.,,,,|||}|||((({{({{,,,...|,|,{{.((((....})))...........{{|{{||.{{(((..{{...{|,,||{.||,...,})),))).}))}}}))}).},})),)))))})}}|||}}..}}}..,.,{((((((((....))))))))}.,.,,..,|,,.}}}.}}|||}}|||||}|}...(((((((..(((....)))...)))))))...,,}}|,||},,(((((..((....(((....)))..))..)))))..|{,.{(((((({,.......({{,,,{,,({,,((.{{..((((({,{,.{|{{{{,(((((......))))).}|.....,,|.,........,}},..(((((((((.....................)))))..((({.....}))))))).....)),)))},))})}},,)),})))},,}..}}))....,(((((.........,},......))}))})}}))}.,,|||||,|||}|||||...,...,,(((((....{{{..,,,,(((.....))))))....)))))..........,,.,.,,..}}),))))){((((((((((((.((((((.((,...)),.{{((.(((.((.(((((((((...(((((((((((((((..(((,,,(((...,},..)),.........)))..))))...)))))))))))............(((..((........)).)))..)))))))))))))))))}))))))..,..{{{{({.{(((.......)))).})))||,,,|,.(({{{{{((,({,....((((........))))......})))}}))),)),,..))).)))))))))}...,,))}}}))))),}}}}....,{{{....))),}).,,))))))),...,)}),,,.,}}}))))

Transcripts

ID Sequence Length GC content
NM_019010.3 NCBI
AGACACACUCUGCCCCAACCAUCCUGAAGCUACAGGUGCUCCCUCCUGG… 1804 nt 0.4579
Summary

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Forensic Context

A study in human keratinocytes demonstrated that X-ray irradiation (5 Gy) induced significant gene expression changes, with the KRT20 being the most strongly upregulated (3.31-fold) and its induction confirmed via RT-PCR and western blot [Koike et al. DOI:10.1269/jrr.46.173]. In human and mouse skin wound healing research, a comprehensive single-cell atlas identified FOSL1 as a critical driver of keratinocyte migration, with validation showing that silencing FOSL1 reduced keratinocyte motility and that EGFR ligands enhanced its expression [Liu et al. DOI:10.1016/j.stem.2024.11.013]. A study in human samples demonstrated that a panel of 19 cytokeratins, including the KRT20, was tested for discriminating epithelial cell origins in forensic swabs, but the KRT20 was found to be only minimally or not at all useful for the specific sexual-assault questions under investigation [Schulz et al. DOI:10.1111/J.1556-4029.2009.01071.X].