Basic Information

Symbol
MRPL13
RNA class
mRNA
Alias
Mitochondrial Ribosomal Protein L13 L13mt RPML13 RPL13 UL13m L13A L13 39S Ribosomal Protein L13, Mitochondrial Large Ribosomal Subunit Protein UL13m MRP-L13 Mitochondrial Large Ribosomal Subunit Protein UL13m
Location (GRCh38)
8:120380761-120445402
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Wound age identification
External database
HGNC NCBI Ensembl OMIM UniProt GTEx

MANE select

Transcript ID
NM_014078.6
Sequence length
1260.0 nt
GC content
0.3960

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-317.20 kcal/mol
Thermodynamic ensemble
Free energy: -341.81 kcal/mol
Frequency: 0.0000
Diversity: 220.14
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
(((((..((((((.(((..(((((((((((((.....)))))(((..(((((((((.......))))))))).)))((((.......))))......(((((.....)))))))))))))((((((((((...((((..(((((.......))))))))).((((((((.....(((...........)))(((((...))))).((((.((((((((((...........((((((.....((((((((((....((((((...((..((((..(((..(((((((((.((((.((((((...((((((((((.((.((..(((....)))..)))).)))))))).))...)))))))).....................(((((...)))))...((((((..((..(......)..))..)))))).............)).)))))))))......((((.((((.((.........(((((...)))))..((((((............((((((......................))))))...........)))))).......)).)))).))))((((((((.((((.((((...((((....................(((((((((....)).))))))).....((((((((((((.((((((((..((((.......))))..))))))))...))..))).)))))))(((((((...(((((((.(((..((((..(((((((((((.......))))))))...(((..(((((((..((((..((((((...((((((.....))))))...)))))).))))..((((.....((((..((((....))))..)))).....)))).((((((............))))))....)))))))..)))((.(((((((((....)))))))))))..............)))..))))...))).))))))))))))))(((((((.(((...((((((((.(((((.((..................))))....))).))))))))...)))))))))).........))))..)))).)))).)......)))))))..)))..))))...))...))))))....)))))..)))))..))))))...........)))))).)))).))))..))))))))...........))))))))))....))).))))))....)))))...........
Thermodynamic Ensemble Prediction
(((((..((((((.(((..(((((((((((((.....)))))(((..(((((((((.......))))))))).)))({(({{....})))}......(((((.....))))}))))))))((((((((((,..((((..{((((.....,,)))}})))).((((({{{,,..,(((...,,,,.,..}})|||||...}}}}}.((((.((((((((((........,..((((((...,.{(((((((((....((((((...{{..((((..(((..(((((((((.((((.((((((...((((((((((.{{.({,.(((....))),,)}}}.)))))))).))...))))))))....{{...........,{{.(((({...})))).,,,..((((...)))).....,..{(((((((......}}}}}}))))).))))))))}......((((.((({.{(......{{.(((((...)))))..|{{{{,............((((((......,...............))))))..,........}))))),,,,...)).}))).))))(((((((,,{(({.,(({...((((,,,{{,,{{{,...,|,,..(((((((((....)).))))))).....((((((((((((.((((((((..((((.......))))..))))))))...)),.})).)))))))(((((((...(((((((.(((..((({..{(({(((((((.......)))))))},{{((({.{,.,}}}},{{{{..((((((...((((((.....))))))...)))))).}}}}.,||||,,|..,,{{{{({{{{{,,|,}|,.},,...,,,|,,,,|||({{......,,,,..}})))}...,,,,||||.....((.(((((((({....})))))))))).,,,}}...,,),,)))..))))...))).))))))))))))))(((((((.(((...((((((((.(((.{,....,,..,,.......,..)).,,,..))).))))))))...))))))))))..,,,,...)))).,)}}),)))),).,,.,.)))))))..)))..))))...}}...))))))....)))))..)))))}.))))))..,........)))))),}))).)))).}))))))))..........,))))))))))....))).))))))....)))))...........

Transcripts

ID Sequence Length GC content
NM_014078.6 NCBI
GAGUGACCCGGCGUGGCUACUAGGAGAAGGACGUACGGUCCUGCUAGUA… 1260 nt 0.3960
Summary

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]

Forensic Context

A study in humans demonstrated that the MRPL13 was among the most highly expressed housekeeping genes with a low coefficient of variation across individuals differing in genetics, environment, age, and gender [Sharma et al. DOI:10.1152/physiolgenomics.00228.2003]. In a forensic context, research in contused rat skeletal muscle validated the MRPL13 as the most stably expressed reference gene for quantitative real-time PCR normalization, with the lowest stability value in NormFinder analysis and the lowest standard deviation in BestKeeper analysis, and it formed the best combination with RPL32 for multi-analysis to improve the accuracy of gene expression studies aimed at estimating wound age [Sun et al. DOI:10.1007/s00414-011-0604-3]. In a separate study in Sprague-Dawley rats, the MRPL13 was validated as a stably expressed reference gene for RT-qPCR normalization in contused skeletal muscle, enabling the accurate quantification of time-dependent wound-healing genes for wound age estimation [Li et al. DOI:10.1007/s00414-023-03095-x].