Basic Information

Symbol
RPL27
RNA class
mRNA
Alias
Ribosomal Protein L27 60S Ribosomal Protein L27 EL27 L27 Large Ribosomal Subunit Protein EL27 DBA16
Location (GRCh38)
17:42998273-43002973
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Time since deposition estimation Postmortem interval inference
External database
HGNC NCBI Ensembl OMIM UniProt GTEx

MANE select

Transcript ID
NM_000988.5
Sequence length
505.0 nt
GC content
0.4733

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-156.3 kcal/mol
Thermodynamic ensemble
Free energy: -167.16 kcal/mol
Frequency: 0.0000
Diversity: 80.94
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
...(((....)))((((((.......((((.((((((((((((((..((((((....((((((((.......((((....))))...)))))))))))).))..))))).))))......((.(((((((((.((.....)).))))))))).))........)))))))))..((((((((((((((..(((....((.......))..)))..))))))))).))))).......((((.......))))((((((...)))))).((((...((((....)))).))))............(((..(((((.((((((((..(((((((....))))..)))...)))))))).)))))..))).(((((((((((..(((((.....).))))...))...))))).)))).(((.((((((((((((((((..((((((((...)).)))))).)))))...))).)))))))))))............)))))).....
Thermodynamic Ensemble Prediction
...(((,,..,}}{{||{|.,,{({{((({{((((((((((((((..({((((....((((((((.,.....((((....)))).,.)))))))))))).))..))))).))))......,(,(((((((((.{{.....)),))))))))}.,,........)))))))))..{{{{{(((((((((..(((....({.......)}..)))..))))))))).|||||.,.....((((.......)))){(((((...)))))).((((..,(({{....)))}.))))....}}}},...{((..(((((.((((((((..{{{((({....})))..)}}...)))))))).)))))..))).((((((((({(..(((((.....).))))...))...))))}}}))).(((.((((((((((((((((..((((((({...})})))))).)))))...)))}}})))))))))............,})))}.....

Transcripts

ID Sequence Length GC content
NM_000988.5 NCBI
AGCGGAAGUGUCCUUCUUUCCUUUUUGCUGGUAGGGCCGGGUGGUUGCU… 505 nt 0.4733
NM_001349921.2 NCBI
AUUCGAAUACUUGAGCUGCCGCGCCCUGCGUUUCCCUCAUGAGCUUCUU… 595 nt 0.4908
NM_001349922.2 NCBI
AGCGGAAGUGUCCUUCUUUCCUUUUUGCUGGUAGGGCCGGGUGGUUGCU… 782 nt 0.5166
Summary

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017]

Forensic Context

A study in Sprague-Dawley rats demonstrated that Rpl27 mRNA was identified as the most stable endogenous control across 25°C and 35°C during the first 24 hours postmortem in cardiac tissue, making it suitable for normalizing qPCR data in early postmortem interval estimation models [Tao et al. DOI:10.1016/j.legalmed.2018.09.004]. A subsequent review confirmed the RPL27's role for early PMI estimation, noting its mention alongside other sensitive markers in foundational studies [Sangwan et al. DOI:10.1016/j.jflm.2021.102251].