Basic Information

Symbol
RPL35A
RNA class
mRNA
Alias
Ribosomal Protein L35a L35A EL33 Cell Growth-Inhibiting Gene 33 Protein Large Ribosomal Subunit Protein EL33 60S Ribosomal Protein L35a GIG33 DBA5
Location (GRCh38)
3:197950000-197956610
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Drug abuse diagnoses
External database
HGNC NCBI Ensembl OMIM UniProt GTEx

MANE select

Transcript ID
NM_000996.4
Sequence length
1234.0 nt
GC content
0.4368

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-355.3 kcal/mol
Thermodynamic ensemble
Free energy: -381.47 kcal/mol
Frequency: 0.0000
Diversity: 333.55
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
....(((((..((((...))))......((((((((((((((..((((((((((....((.....((((.(((.(((((((......(((((.......)))))...(((...((((((..........)))).))...)))((((........))))((((((..(((((((..(((......)))..)))))))...))))))((((.........)))).........)))))))..))).)))).....))........(((((....))))))))..)))..))))..)))..((((....(((((((...)))))))..))))...((((((((.(((((((((((..(.((((.....((((...(((...((..(((.....(((((...........)))))..........(((((...((.((((((((((...(((((..........)))))..(((((..(((.....((.....))....)))....)))))..(((((((((((.((((..((((((((...(((.((..((((((....))))))((((...(((((((((((((((((((..((((......(((((..(((.....)))..)))))......))))..((..((((.(.(((((.((.(((((.........(((((((((.((((.........)))))))))))))))))).)).)))))).))))..)).....)))))........)))))).))))))))(((((.......))))))))).((((((((.((......))))))..)))).)))))))))))))....)))).)))))))))))))))))....))))))))))).(((((((((((...((...))...)))))))......))))...)))..)).....)))....)))).....)))).)..)))))))))))...((((...((((.(((((((.((((((...((((((....((((((........))))))..................(((((.....(((...(((......((((((((((.......))))).)))))......)))...)))...))))))).))))...)))))).))))))).))))...))))..))))))))......)))))))))))....((((((((((.(((...))).)).)))))))).......))))).....
Thermodynamic Ensemble Prediction
.....,,,,..((({...})))...,..((((((((((((((..((((((((({....((.....((((.(((.((((((({{{...||||,{{{(((({,,,|(((((({,,{((((.....||.,,{{((((.....))))|||,,......}||||||,,...(((((({..(((......)))..}))))))..))))).))))))}}.......))))}}}}....|})))))..))).)))|,....))........(((((....)))))||...|}}..))))..))).,(({{.,,.({(((((,,,|||||||..}}||..,((({{{{,..,|||..}|{{,||||,}||,|||}|||||,{,,|||{|.||||....||,{{{...,,......{{{||.,,.......|||,|}}}}||{{{{||||||{{{|{{|||,,||,,,...,,,.,.{{{{{..,{{.,{,,((.....))...,}}}.,,,)))||..(((((((((((.((((..((((((((...(((.{{..((((((....))))))(((({{,((((((((((((((((,({.{((((......(((((..(((.....)))..))))),,},,.))))..((..((((.(.(((((.((.(((((.........(((((((((.((((.........)))))))))))))))))).)).)))))).))))..))..{{,..,,))},)}.,)))))))))))))))),{{{{.......))))))))).((((((({{((......)))))},.)))).}})))))))))))....)))).)))))))))))..}|,|{{.{|{({|,(({.((((((((((((({{{,,,...|,.{(((((.....})))))...||{{...},,......{((.(((((.......((((((((((....,,{{.((((.((((.(((((((((....,.{{{.,{..,......},}}}}....))))))))).)))).)))}.,,.,.))))).)))))))))).)))...,}))}}.,,)).)))))))),{{|||{|,|,{|{{|.,|||||{.,....))}}))}},))))),)}})))})))}}},,..))))..})).,))))))))......)))))))))))....(((((((({{.(((...))).}}.)))))))).......}}))).....

Transcripts

ID Sequence Length GC content
NM_000996.4 NCBI
CUUCUCUUACCGCCAUCUUGGCUCCUGUGGAGGCCUGCUGGGAACGGGA… 1234 nt 0.4368
NM_001316311.2 NCBI
CUUCUCUUACCGCCAUCUUGGCUCCUGUGGAGGUGAGUGAAGGGUCUGC… 1312 nt 0.4436
Summary

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Oct 2015]

Forensic Context

A study in human postmortem brain tissue demonstrated that the RPL35A mRNA was hypomethylated in the nucleus accumbens of subjects with alcohol use disorder compared to matched controls, with a mean methylation level of 0.232 in cases versus 0.538 in controls [Liu et al. DOI:10.3390/genes13060958].