Basic Information

Symbol
AIF1
RNA class
mRNA
Alias
Allograft Inflammatory Factor 1 AIF-1 IBA1 Ionized Calcium-Binding Adapter Molecule 1 IRT-1 Interferon Gamma Responsive Transcript Em:AF129756.17 Protein G1 IRT1 G1
Location (GRCh38)
6:31615217-31617021
UCSC Genome Browser Search in Marker Scope
Forensic tag(s)
Mechanical injury analysis Wound age identification
External database
HGNC NCBI Ensembl OMIM UniProt GTEx

MANE select

Transcript ID
NM_001623.5
Sequence length
655.0 nt
GC content
0.4779

Secondary Structure

Generated by RNAfold
Minimum free energy (MFE) structure:
Secondary structure that contributes a minimum of free energy.
Ensemble properties:
Thermodynamic properties of the Boltzmann ensemble.
Minimum free energy
-186.60 kcal/mol
Thermodynamic ensemble
Free energy: -199.01 kcal/mol
Frequency: 0.0000
Diversity: 154.70
MFE Structure Visualization
Structure Prediction
MFE Structure Prediction
.(((((((.....(.((((.(((......((((.((((........))))))))((((.((..(((((((((...(((.(((((((..(((.(....).))).....(((((((........(((((...(((((...((((((......))))))...)))))(((.(.((....)).).)))..)))))....(((((((.((......))..)).))))).(((.((((....((.((((((((............))))))))..))....))))...)))((((....))))..)))))))......))))))))))......(((((.....))))).....(((((..........((((((......))))))))))).))))))))).))))))..(((...((((((((((((..((.((........)).))))))))........((((........))))......))))))..)))....(((....)))))))))))..)).)))))((((((......((((.......))))...))))))....(((..((.(((((((((......(((....)))....)))))......((((..((....))..)))).............)))).)).))).
Thermodynamic Ensemble Prediction
.(((((((..,..{.((((.(((.,....((((.((((........)))))))),{{||((.,(((((((((...(((.((((((({.({(,,,,..,.))),....(((((((.....{{{({,,...,(((((...((((((......))))))...))))){||,,.((...,||.,.}}}..}}})}....((((({{..{.,....}},.)}.))))).(((.((((....((.((((((((............))))))))..))....))))...)))|{{,,,..)})),,)))))))....}.}))))))))).....{((({(.,,.,)}}}}.....(((((,.........{(((((......))))))))))).))))))))}.|||||}..(((,,.{{{{|||||{{{..||,|{||....,.}),)))}||||........((((........))))......}})}),..}))....(((....)))))))))))..))}}}))|((((((......((((.......))))...)))))),.,,(({..({.(((((((((......(((....)))....)))))......{(({..{{....))..))}}.,,..........}))),)).))).

Transcripts

ID Sequence Length GC content
NM_001318970.2 NCBI
AGGCUUCUGAGAAGACUGGUGGGAGAGAAGGAGAGCCUGCAGACAGAGG… 594 nt 0.4714
NM_001623.5 NCBI
AGGCUUCUGAGAAGACUGGUGGGAGAGAAGGAGAGCCUGCAGACAGAGG… 655 nt 0.4779
NM_032955.3 NCBI
CUCCUUCCACGUUGUCUCCUCCACCUAGCAGUUGGUUGGCAACCCCUUC… 518 nt 0.4768
Summary

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

Forensic Context

A study in human samples using bulk and single-cell/nucleus RNA sequencing identified the AIF1 as a marker for manual annotation of macrophage clusters in a carotid atherosclerosis dataset [Weiqi Xue et al. DOI:10.3233/JAD-230559]. A study in mice demonstrated that the AIF1 is a marker used to identify immune cells including macrophages, and immunohistochemistry using this marker showed modest increases in gliosis in the meninges 24 hours post-traumatic brain injury [Bolte et al. DOI:10.7554/eLife.81154]. In rats, subcutaneous injection of leptin and fractalkine induced significant recruitment of Iba1-immunopositive macrophages in the skin compared to control, validating its use for identifying macrophage infiltration in a burn injury model [Friston et al. DOI:10.1242/dmm.042713].